Patent · US Active

Digital assays with a reporter for amplicon length

US9702822B2 · kind B2 · utility

6Cited by

0References

8Claims

0Family size

Assignee

Bio-Rad Laboratories, Inc. · US

Inventors

Claudia Litterst · Walnut Creek, US
Austin So · Pleasanton, US
Duc Do · Brooklyn, US

Key dates

Filing date	Aug 22, 2013
Grant date	Jul 11, 2017
Priority date	—
Expiry date	Mar 26, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6858
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.