Methods for creating doubled haploid maize embryos using oil matrices
US9706723B2 · kind B2 · utility
1Cited by
5References
2Claims
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Key dates
| Filing date | Jul 7, 2015 |
| Grant date | Jul 18, 2017 |
| Priority date | — |
| Expiry date | Jul 7, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6895
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.