Endoribonuclease compositions and methods of use thereof

US9708646B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Rachel E. Haurwitz · Kensington, US
• Jennifer A. Doudna · Berkeley, US
• Blake Wiedenheft · Bozeman, US
• Martin Jinek · Berkeley, US

Key dates

• Filing date: Feb 10, 2017
• Grant date: Jul 18, 2017
• Expiry date: Feb 10, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6816
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.