Detection of nucleic acid amplification in a porous substrate
US9714447B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 19, 2013 |
| Grant date | Jul 25, 2017 |
| Priority date | — |
| Expiry date | Dec 2, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.