Methods and materials for capture antibody targeted fluorescent in-situ hybridization (CAT-FISH)

US9714943B2 · kind B2 · utility

Assignee

• University of South Florida · US

Inventors

• Joyce Marie Stroot · Parker, US
• Peter George Stroot · Lutz, US
• Kelly M. Leach · Tampa, US
• Daniel V. Lim · Tampa, US

Key dates

• Filing date: Dec 23, 2011
• Grant date: Jul 25, 2017
• Expiry date: Dec 23, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/56911
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The subject invention concerns materials and methods for detecting a target cell in a population. Methods of the invention comprise internally labeling cells via fluorescence in situ hybridization (FISH) using probes that target rRNA, followed by binding of capture antibodies targeted (CAT) for specific cell surface epitopes on the target cells. In one embodiment, the target cells are bacterial cells.