Production of omega-amino fatty acids

US9719117B2 · kind B2 · utility

Assignee

• EVONIK DEGUSSA GMBH · DE

Inventors

• Steffen Schaffer · Herten, DE
• Jasmin Gielen · Bochum, DE
• Mirja Wessel · Bochum, DE
• Hans-Georg Hennemann · Marl, DE
• Harald Haeger · Thedinghausen, DE
• Thomas Haas · Munster, DE
• Wilfried Bluemke · Straubing, DE

Key dates

• Filing date: Dec 18, 2013
• Grant date: Aug 1, 2017
• Expiry date: Dec 18, 2033

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/582
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.