Patent · US Active

Method for determining the degree of methylation of defined cytosines in genomic DNA in the sequence context of 5′-CpG-3′

US9719131B2 · kind B2 · utility

2Cited by

2References

7Claims

0Family size

Assignee

EPIGENOMICS AG · DE

Inventors

Alexander Olek · Berlin, DE
Christian Piepenbrock · Berlin, DE
Kurt Berlin · Stahnsdorf, DE
David Guetig · Berlin, DE

Key dates

Filing date	Jul 30, 2010
Grant date	Aug 1, 2017
Priority date	—
Expiry date	Jul 30, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/154
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method is described for the detection of the degree of methylation of a specific cytosine in the sequence context 5′-CpG-3′ of a genomic DNA sample. In the first step, the genomic DNA is chemically treated in such a way that the cytosine bases are converted to uracil, but not the 5-methylcytosine bases. Then segments of the genomic DNA which contain the said specific cytosine are amplified, whereby the amplified products are given a detectable label and in the following steps the extent of hybridization of the amplified products on two classes of oligonucleotides is determined by detection of the label of the amplified products, and a conclusion is made on the extent of methylation of said specific cytosine in the genomic DNA sample from the ratio of the labels detected on the two classes of oligonucleotides as a consequence of the hybridization.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.