Massive parallel method for decoding DNA and RNA

US9719139B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Zengmin Li · Great Neck, US
• John Robert Edwards · Nobleton, CA
• Yasuhiro Itagaki · New York, US

Key dates

• Filing date: Dec 15, 2016
• Grant date: Aug 1, 2017
• Expiry date: Dec 15, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.