Animal product-free culture medium and a process for producing a supernatant of clostridium comprising one or more collagenolytic and gelatinolytic proteases
US9725692B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 2013 |
| Grant date | Aug 8, 2017 |
| Priority date | — |
| Expiry date | May 29, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/24003
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention refers to a culture medium and a process for producing Clostridium collagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium for C. histolyticum comprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant of Clostridium histolyticum liquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of the Clostridium cultures described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.