Expression method

US9725704B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Tobias Kueppers · Rheydt, DE
• Victoria Steffen · Emmelshausen, DE
• Renee Charlott Eichstadt · Cologne, DE
• Stefan Evers · Mettmann, DE
• Karl-Heinz Maurer · Erkrath, DE
• Johannes Bongaerts · Dormagen, DE
• Hendrik Hellmuth · Darmstadt, DE
• Thomas Weber · Dormagen, DE
• Timothy O'Connell · Fürstenfeldbruck, DE

Key dates

• Filing date: Jan 29, 2013
• Grant date: Aug 8, 2017
• Expiry date: Jan 29, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.