PIIINP neo-epitope assay
US9726674B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 2014 |
| Grant date | Aug 8, 2017 |
| Priority date | — |
| Expiry date | Apr 15, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/7052
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a monoclonal antibody specifically reactive with a C-terminal neo-epitope of PIIINP comprised in a C-terminal amino acid sequence CPTGXQNYSP-COOH (SEQ ID NO: 4) in which X is Gly or Pro, and where the monoclonal antibody does not recognize or bind an elongated version of the C-terminal amino acid sequence CPTGXQNYSPQZ-COOH (SEQ ID NO: 5), in which Z is absent or is one or more amino acids of the sequence of collagen type III. Also provided is a method of immunoassay for detecting in a biological sample the C-terminal neo-epitope of PIIINP generated by N-protease cleavage of intact type III procollagen, by contacting the sample with the monoclonal antibody, and determining the amount of binding of the antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.