Protein retrosplicing enabled by a double ligation reaction
US9731029B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | May 21, 2013 |
| Grant date | Aug 15, 2017 |
| Priority date | — |
| Expiry date | Aug 11, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/06
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Proteins containing a C-terminal thioester are important intermediates in semi-synthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. The invention involves, in some aspects a method, utilizing Sortase A, for preparation of recombinant proteins containing a C-terminal αthioester. This new method for double ligatation is useful for synthesizing new or naturally occurring molecules such as a protein thioester.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.