Methods of modifying transcriptional regulatory networks in stem cells
US9732128B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 21, 2011 |
| Grant date | Aug 15, 2017 |
| Priority date | — |
| Expiry date | Jul 17, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/603
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The vast differentiation potential of human embryonic and induced pluripotent stem cells, including their potential to cascade through all of the somatic cell lineages and to display the complete transcriptional regulatory network of human biology, has generated interest in deriving scalable, purified, and identified cell types and methods of discovering the precise structure of the human regulatory network. However, the innate capacity of pluripotent cells to display all these lineages is not necessarily reflected during their culture in vitro. The clonal isolation and propagation of progenitors greatly facilitates the generation of highly purified and identified formulations for research and therapeutic purposes. Nevertheless, other cell types have yet to be isolated and propagated from normal cells and methods of isolating said novel cell types as well as methods for introducing perturbations into the transcriptional regulatory network in order to construct a computer model of the entire human transcriptional regulatory network would greatly benefit basic research as well as manufacturing technology for cell-based therapies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.