Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
US9732319B2 · kind B2 · utility
5Cited by
45References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 19, 2011 |
| Grant date | Aug 15, 2017 |
| Priority date | — |
| Expiry date | Dec 14, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides cell culture conditions for culturing stem cells, including feeder-free conditions for generating and culturing human induced pluripotent stem cells (iPSCs). More particularly, the invention provides a culture platform that allows long-term culture of pluripotent cells in a feeder-free environment; reprogramming of cells in a feeder-free environment; single-cell dissociation of pluripotent cells; cell sorting of pluripotent cells; maintenance of an undifferentiated status; improved efficiency of reprogramming; and generation of a naïve pluripotent cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.