Patent · US Active

Formulation of sugar solutions for continuous ultracentrifugation for virus purification

US9732327B2 · kind B2 · utility

2Cited by

2References

27Claims

0Family size

Assignee

Baxalta Incorporated · CH

Inventors

Manfred Reiter · Wien, AT
Leopold Grillberger · Wien, AT
Wolfgang Mundt · Wien, AT
Artur Mitterer · Orth an der Donau, AT
Horst Schafhauser · Wien, AT

Key dates

Filing date	Jan 23, 2015
Grant date	Aug 15, 2017
Priority date	—
Expiry date	Jan 23, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2760/16252
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.