Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof

US9732353B2 · kind B2 · utility

Assignees

• DOW AGROSCIENCES LLC · US
• M.S. Technologies, L.L.C. · US

Inventors

• Thomas Hoffman · Zionsville, US
• Ning Zhou · East Hanover, US
• Dayakar Pareddy · Carmel, US
• Yunxing C. Cui · Carmel, US
• Dawn M. Parkhurst · Westfield, US
• Nathan Bard · Edgerton, US
• Sandra G. Toledo · Zionsville, US
• Gregory A. Bradfisch · San Diego, US
• Bruce Held · Ames, US
• Vaithilingam Sekar · Ames, US
• Lauren Clark · Whitestown, US
• Sean M. Russell · Carmel, US
• Kelly Ann Smith · Lebanon, US
• Yang Wang · Palo Alto, US
• Terry R. Wright · Carmel, US

Key dates

• Filing date: Jul 13, 2012
• Grant date: Aug 15, 2017
• Expiry date: Feb 22, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y205/01019
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to soybean event pDAB8264.42.32.1 and includes novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TAQMAN PCR assays for the detection of Event pDAB8264.42.32.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.