General strategy for antibody library screening

US9733240B2 · kind B2 · utility

Assignee

• Merck Patent GmbH · DE

Inventors

• Stefan Becker · Darmstadt, DE
• Tim Heiseler · Roßdorf, DE
• Alexander Maass · Ludwigsburg, DE
• Harald Kolmar · Göttingen, DE

Key dates

• Filing date: Dec 14, 2012
• Grant date: Aug 15, 2017
• Expiry date: Sep 8, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B30/08
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A generally applicable method for the selective covalent attachment of a reporter molecule to a replicating entity that allows one to obtain specific binders from a single round of library screening is disclosed. For example, selective biotinylation of phage particles and yeast cells displaying a binder to any given target can be achieved via application of a coupled enzyme reaction that includes a peroxidase, an oxidase and a catalase.