Method and a device for cross-talk correction of measured intensities
US9736555B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 8, 2014 |
| Grant date | Aug 15, 2017 |
| Priority date | — |
| Expiry date | Apr 13, 2034 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH04Q2011/0049
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for cross-talk correction of intensities measured on mutually separate detection wavelength bands is presented. Each detection wavelength band relates to one of analyte-specific probe-populations contained by a sample to be analyzed. Each probe-population is capable of emitting a first signal component and a second signal component whose spectra have maxima at different wavelengths and at least the first signal component is dependent on presence of analyte detectable with that probe-population. Cross-talk corrected intensities are computed on the basis of a) the intensities measured on the detection wavelength bands, b) a value indicative of intensity occurring on an auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and c) pre-determined cross-talk parameters. For example in con-junction with FRET-based assays, the dependency of a background signal on the percentage of hybridized probes can be taken into account in the cross-talk correction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.