Method of extracting organelles from cells
US9738871B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 3, 2015 |
| Grant date | Aug 22, 2017 |
| Priority date | — |
| Expiry date | Apr 8, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of extracting organelles from cells includes following process. A plurality of cells and a solution are mixed uniformly to obtain a mixture. The mixture is loaded into a container. The container is placed in a milling device comprising a miller and a mechanical power source connected to the miller. The miller is immersed into the mixture within the container. The mixture is milled by the miller to obtain a homogenized mixture, wherein a milling parameter is provided to the milling device, and the mechanical power source drives the miller according to the milling parameter. The homogenized mixture is centrifuged to obtain a supernatant. The supernatant is centrifuged to obtain a precipitate with a plurality of organelles. In the process that the mixture is milled by the miller, at least a part of the miller is kept in the mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.