Seed-specific expression vector and its construction methods and applications

US9745362B2 · kind B2 · utility

Assignees

• Shengjun An · CN
• Kunsheng Wang · CN
• Xiqing Chai · CN

Inventors

• Shengjun An · Shijiazhuang, CN
• Xiqing Chai · Shijiazhuang, CN
• Kunsheng Wang · Kunming, CN
• Tiemei Shao · Shijiazhuang, CN
• Zhan Jiao · Shijiazhuang, CN
• Xin Wen · Rochester, US
• Xue Li · Beijing, CN
• Pei-Qi Liu · Richmond, US
• Haigang Lu · Shijiazhuang, CN
• Yunyu Chen · Shijiazhuang, CN
• Liangyuan Hu · Beijing, CN
• Haimin Xu · Shanghai, CN
• Chenggang Yu · Beijing, CN

Key dates

• Filing date: Dec 9, 2010
• Grant date: Aug 29, 2017
• Expiry date: May 28, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8257
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.