Detection of PNA clamping
US9745633B2 · kind B2 · utility
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2References
16Claims
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Assignee
Inventors
Key dates
| Filing date | Aug 28, 2014 |
| Grant date | Aug 29, 2017 |
| Priority date | — |
| Expiry date | Aug 20, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods and systems for detecting sequence variants in a sample nucleic acid. Methods include PNA clamping/PCR using primers with binding moieties followed by capture and detection of amplicons on a solid support. Systems include PCR reagents with primers having binding moieties, a PNA clamping probe, a solid support capable of capturing the PCR amplicons incorporating the binding moieties, and a detector device.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.