Alternative nucleic acid molecules containing reduced uracil content and uses thereof

US9751925B2 · kind B2 · utility

Assignee

• ModernaTX, Inc. · US

Inventors

• Stephen Hoge · Brookline, US
• William Issa · Dedham, US
• Edward J. Miracco · Arlington, US
• Jennifer Nelson · Brookline, US
• John Reynders · Newton, US
• Matthew G. Stanton · Marlton, US

Key dates

• Filing date: Dec 4, 2015
• Grant date: Sep 5, 2017
• Expiry date: Dec 4, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y113/12007
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them. In some aspects, the disclosure provides mRNA wherein the uracil content has been modified and which may be particularly effective for use in therapeutic compositions, because they may benefit from both high expression levels and limited induction of the innate immune response. In some aspects, the disclosure provides methods for the production of pharmaceutical compositions including mRNA without reverse phase chromatography.