Methods and compositions for amplification and detection of MicroRNAs
US9752180B2 · kind B2 · utility
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Key dates
| Filing date | Nov 11, 2013 |
| Grant date | Sep 5, 2017 |
| Priority date | — |
| Expiry date | Jun 11, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and system for amplifying non-coding RNA, microRNA, and small polynucleotide sequences through the generation of a pool of signature sequences to the target sequences. The target sequences can be amplified through DNA synthesis, RNA synthesis, or the combination of DNA and RNA synthesis. The amplification of signature sequences provides an efficient and reproducible mechanism to determine the presence or absence of the target miRNAs, to analyze the quantities of the miRNAs, and for miRNA profiling. The method may also be used for screening for unknown non-coding RNAs, including novel miRNAs.
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