Single cell capture with capture chips

US9757707B2 · kind B2 · utility

Assignee

• Takara Bio USA, Inc. · US

Inventors

• Syed A. Husain · Milpitas, US
• Bradley L. Griswold · San Jose, US
• Michael Slater · Modesto, US
• Patricio A. Espinoza Vallejos · Mountain View, US
• Jude Dunne · Menlo Park, US
• Glenn Hein · Fremont, US
• Maithreyan Srinivasan · Palo Alto, US

Key dates

• Filing date: Oct 28, 2015
• Grant date: Sep 12, 2017
• Expiry date: Oct 28, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N1/405
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and/or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.