Recombinant cell surface capture proteins

US9758592B2 · kind B2 · utility

Assignee

• REGENERON PHARMACEUTICALS, INC. · US

Inventors

• Dipali Deshpande · White Plains, US
• Gang Chen · Sammamish, US
• Darya Burakov · Tarrytown, US
• James P. Fandl · Lagrangeville, US
• Thomas H. Aldrich · Yorktown Heights, US
• Vishal Kamat · Bergenfield, US

Key dates

• Filing date: Nov 14, 2013
• Grant date: Sep 12, 2017
• Expiry date: Apr 30, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/92
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and/or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).