Filter-based method for efficient capture of lysis of suspended cells
US9758755B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 23, 2015 |
| Grant date | Sep 12, 2017 |
| Priority date | — |
| Expiry date | Oct 23, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M29/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.