Patent · US Active

Cell surface display of polypeptide isoforms by stop codon readthrough

US9758779B2 · kind B2 · utility

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8Claims

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Assignee

Novartis AG · CH

Inventors

Thomas Jostock · Neuenburg am Rhein, DE
Hans-Peter Knopf · Schallstadt, DE
Burkhard Wilms · Weil am Rhein, DE
Audrey Nommay · Blotzheim, FR

Key dates

Filing date	Aug 28, 2009
Grant date	Sep 12, 2017
Priority date	—
Expiry date	Jul 9, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/035
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The application describes a method of selecting mammalian host cells that express a polypeptide of interest with high yield. The host cells contain an expression cassette with a first polynucleotide encoding a polypeptide of interest, at least one leaky stop codon located downstream of the first polynucleotide and a second polynucleotide located downstream of the leaky codon encoding an immunoglobulin transmembrane anchor comprising a cytoplasmic domain. The host cells are cultivated to allow expression of the polypeptide of interest such that some of the polypeptides of interest are expressed as fusion proteins displayed on the cell surface. High producing cells are then selected based on the presence or amount of the displayed fusion polypeptides.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.