Bioluminescence resonance energy transfer between bioluminescent proteins and semiconductive nanomaterials
US9758808B1 · kind B1 · utility
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18Claims
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Key dates
| Filing date | Nov 6, 2013 |
| Grant date | Sep 12, 2017 |
| Priority date | — |
| Expiry date | Dec 8, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2521/543
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A bioluminescence energy transfer (BRET) nanosystem having semiconductive quantum rods (QRs) bound by firefly luciferase Photinus pyralis (Ppy) for improved conversion of chemical energy to light, such as in solid-state lighting, near-infrared imaging systems, and in vivo infrared imaging. The nanosystems are formed by synthesizing CdSe/CdS or CdSe/CdS/ZnS quantum rods, rendering the dots hydrophilic and colloidially stable with a facile His-capping, incubating with a Ppy variant (PpyGRTS) at increasing loading ratios, and adding an excess of the luciferin (LH2) substrate to the PpyGRTS-QRs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.