Chemically defined albumin-free conditions for cardiomyocyte differentiation of human pluripotent stem cells
US9765299B2 · kind B2 · utility
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Key dates
| Filing date | Sep 10, 2015 |
| Grant date | Sep 19, 2017 |
| Priority date | — |
| Expiry date | Sep 10, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/45
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for generating high-yield, high-purity cardiomyocyte progenitors or cardiomyocytes from pluripotent cells are described. Wnt/β-catenin signaling is first activated in pluripotent cells, by, for example, inhibiting Gsk-3 to obtain a first population of cells. Wnt/β-catenin signaling is then inhibited in the first cell population to induce cardiogenesis. One or more of these steps is performed under defined, albumin-free culture conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.