Method and system for imaging a molecular strand
US9766180B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 2, 2014 |
| Grant date | Sep 19, 2017 |
| Priority date | — |
| Expiry date | Jun 2, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.