Biosensors engineered from conditionally stable ligand-binding domains

US9766255B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• University of Washington · US

Inventors

• George M. Church · Brookline, US
• Justin Feng · Cambridge, US
• Daniel J. Mandell · Brookline, US
• David Baker · Ann Arbor, US
• Stanley Fields · East Setauket, US
• Benjamin Ward Jester · Seattle, US
• Christine Elaine TINBERG · Seattle, US

Key dates

• Filing date: Jan 12, 2016
• Grant date: Sep 19, 2017
• Expiry date: Jan 12, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.