Methods and apparatus for synthesizing nucleic acid
US9771613B2 · kind B2 · utility
3Cited by
18References
23Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Aug 18, 2015 |
| Grant date | Sep 26, 2017 |
| Priority date | — |
| Expiry date | Aug 18, 2035 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/582
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template. Because the nucleotide analogs have an unmodified 3′ OH, i.e., as found in “natural” deoxyribose and ribose molecules, the analogs result in natural polynucleotides suitable for incorporation into biological systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.