Method of purifying HIV/SIV Nef from exosomal fusion proteins
US9777042B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Dec 15, 2011 |
| Grant date | Oct 3, 2017 |
| Priority date | — |
| Expiry date | Jun 1, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/566
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.