Organic small hairpin RNAs

US9777277B2 · kind B2 · utility

Assignee

• BRANDEIS UNIVERSITY · US

Inventors

• Nelson Lau · Watertown, US
• Mei Zeng · Newton, US
• Suzanne Paradis · Lexington, US
• Marissa S. Kuzirian · Waltham, US

Key dates

• Filing date: Jan 29, 2014
• Grant date: Oct 3, 2017
• Expiry date: Jan 29, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2320/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are improved shRNA molecules, termed “organic shRNA” (OshRNA), that incorporate certain structural features that increase the likelihood that the desired guide strand is produced while reducing accumulation of passenger strands that might contribute to off-target effects. Also provided herein are nucleic acids encoding OshRNAs, kits, cells, and transgenic animals comprising such nucleic acids, as well as methods of making and using OshRNAs and/or nucleic acids encoding OshRNAs.