Methods for phenotyping of intact whole tissues
US9778155B2 · kind B2 · utility
45Cited by
5References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 4, 2015 |
| Grant date | Oct 3, 2017 |
| Priority date | — |
| Expiry date | Nov 4, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/305
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.