Methods for measuring the efficacy of a stain/tissue combination for histological tissue image data
US9779503B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 28, 2017 |
| Grant date | Oct 3, 2017 |
| Priority date | — |
| Expiry date | Feb 28, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method measures the efficacy of a stain/tissue combination for histological tissue image data comprising a set of pixels. The measures of efficacy comprise a statistical measure of clustering performance of the different stain/tissue combinations in the Maxwellian Chromaticity plane, and a representative density of the stain/tissue combinations in the density maps. The method captures histological tissue image data using a light absorbent stain; transforms the histological tissue image data to optical density data; projects the optical density data to the Maxwellian Chromaticity plane; identifies at least one reference color using a statistical technique; inverts the reference color to form a color mixing matrix; derives a density map; derives a statistical measure for the clustering performance of the different stain/tissue combinations in the Maxwellian Chromaticity plane; and finds a representative density of the stain/tissue combinations in the density maps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.