Automated staining and segmentation quality control

US9785848B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• Brion Daryl Sarachan · Schenectady, US
• Alberto Santamaria-Pang · Guilderland, US
• Yousef Al-Kofahi · Niskayuna, US
• Edward Moler · Aliso Viejo, US
• Raghav Krishna Padmanabhan · Aliso Viejo, US
• Qing Li · San Diego, US

Key dates

• Filing date: Apr 30, 2015
• Grant date: Oct 10, 2017
• Expiry date: Oct 22, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G06V20/695
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosed subject matter relates to an automated determination the stain quality and segmentation quality of a tissue sample. By way of example, separate image data is acquired of an unstained form of a biological specimen, the biological specimen stained with a nuclei marker and the biological specimen stained with a segmentation marker. A correlation map (Cr) from the separate image data and a ridgeness map (Pr) from the image data of the biological specimen stained with a segmentation marker are each determined. A staining quality score and segmentation quality score are then determined from the correlation map (Cr) and the ridgeness map (Pr).