Thin-ice grid assembly for cryo-electron microscopy
US9786469B2 · kind B2 · utility
2Cited by
6References
13Claims
0Family size
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Key dates
| Filing date | Aug 10, 2016 |
| Grant date | Oct 10, 2017 |
| Priority date | — |
| Expiry date | Aug 10, 2036 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J2237/2004
- WIPO fieldElectrical machinery, apparatus, energy
- WIPO sectorElectrical engineering
Abstract
A grid assembly for cryo-electron microscopy may be fabricated using standard nanofabrication processes. The grid assembly may comprise two support members, each support member comprising a silicon substrate coated with an electron-transparent silicon nitride layer. These two support members are positioned together with the silicon nitride layers facing each other with a rigid spacer layer disposed therebetween. The rigid spacer layer defines one or more chambers in which a biological sample may be provided and fast frozen with a high degree of control of the ice thickness.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.