Compositions containing, methods involving, and uses of non-natural amino acid linked dolastatin derivatives

US9796754B2 · kind B2 · utility

Assignee

• Ambrx, Inc. · US

Inventors

• Zhenwei Miao · San Diego, US
• Kyle Atkinson · San Diego, US
• Sandra Biroc · Alameda, US
• Timothy Buss · Carlsbad, US
• Melissa Neal · Vista, US
• Vadim Kraynov · San Diego, US
• Robin Marsden · San Diego, US
• Jason Pinkstaff · Novato, US
• Lillian Skidmore · San Diego, US
• Ying Sun · Waltham, US
• Agnieszka Szydlik · San Francisco, US
• Delia Ianina Lopez De Valenta · Poway, US

Key dates

• Filing date: May 24, 2012
• Grant date: Oct 24, 2017
• Expiry date: May 4, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K5/06043
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed herein are non-natural amino acids and dolastatin analogs that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The dolastatin analogs can include a wide range of possible functionalities, but typically have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are non-natural amino acid dolastatin analogs that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such dolastatin analogs. Typically, the modified dolastatin analogs include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid dolastatin analogs and modified non-natural amino acid dolastatin analogs, including therapeutic, diagnostic, and other biotechnology use.