Methods for transforming tarwi and for producing molecular farming products in transgenic tarwi seed
US9796981B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 17, 2013 |
| Grant date | Oct 24, 2017 |
| Priority date | — |
| Expiry date | Aug 3, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y305/04004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.