Patent · US Active

Expression vectors for an improved protein secretion

US9803183B2 · kind B2 · utility

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1References

12Claims

0Family size

Assignee

BASF SE

Inventors

Christian Degering · Erkrath, DE
Thorsten Eggert · Hattingen, DE
Stefan Evers · Mettmann, DE
Karl-Heinz Maurer · Erkrath, DE
Johannes Bongaerts · Dormagen, DE

Key dates

Filing date	May 25, 2012
Grant date	Oct 31, 2017
Priority date	—
Expiry date	Jan 27, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/75
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.