Means and methods for the enzymatic production of L-methionine from O-phospho-L-homoserine and methanethiol

US9803225B2 · kind B2 · utility

Assignee

• ADISSEO FRANCE S.A.S. · FR

Inventors

• Muriel Merkamm · Les Ulis, FR
• Dominique Louis · Le Chardonnet, FR
• Karine Jaillardon · Saint-Michel-sur-Orge, FR
• Dominique Thomas · Gif-sur-Yvette, FR
• Philippe Marliere · Paris, FR

Key dates

• Filing date: Oct 25, 2013
• Grant date: Oct 31, 2017
• Expiry date: Oct 21, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y205/01048
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3PO4. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyze the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4.