Microarray-based assay integrated with particles for analyzing molecular interactions

US9803236B2 · kind B2 · utility

Assignees

• TSINGHUA UNIVERSITY · CN
• CAPITALBIO CORPORATION · CN

Inventors

• Guanbin Zhang · Beijing, CN
• Guangxin Xiang · Beijing, CN
• Wanli Xing · Beijing, CN
• Jing Cheng · Beijing, CN

Key dates

• Filing date: Aug 6, 2010
• Grant date: Oct 31, 2017
• Expiry date: Aug 6, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/155
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way.