Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
US9803238B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 26, 2013 |
| Grant date | Oct 31, 2017 |
| Priority date | — |
| Expiry date | Aug 17, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0409
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.