Method for producing parvovirus having high infectivity titer
US9809800B2 · kind B2 · utility
1Cited by
4References
15Claims
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Key dates
| Filing date | Sep 5, 2013 |
| Grant date | Nov 7, 2017 |
| Priority date | — |
| Expiry date | Sep 5, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14351
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for stably and easily producing a parvovirus having a high infectivity titer is provided. The problem is solved by a method for producing a parvovirus having an infectivity titer as high as 108 TCID50/mL or more in a culture supernatant, comprising the steps of inoculating a seed virus of the parvovirus into a culture substrate comprising host cells having a cell density of 1/500 to 1/20 of the cell density of the host cells confluently grown and a medium at a multiplicity of infection of 0.0001 to 0.1, culturing for a period of 5 to 11 times the doubling time of the host cells, and recovering a culture supernatant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.