Method for concentrating cells that are genetically altered by nucleases
US9809839B2 · kind B2 · utility
1Cited by
0References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 22, 2012 |
| Grant date | Nov 7, 2017 |
| Priority date | — |
| Expiry date | Feb 22, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/50
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a reporter construct and method for identifying or enriching the cells, wherein a specific endogenous nucleotide sequence is cleaved by a specific nuclease or modified by such cleavage; a host cell comprising the reporter construct; and a system for monitoring a nuclease activity. The reporter system of the present invention is simple and non-invasive, and allows for an efficient enrichment of the gene-modified cells. Therefore, the present invention will promote the application of a nuclease in the field of gene therapy and genetic engineering as well as basic research.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.