Detection of nucleic acid sequence differences by comparative genomic hybridization

US9809841B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Donna G. Albertson · Lafayette, US
• Daniel Pinkel · Lafayette, US
• Yevgeniya Fridlyand · San Francisco, US
• Bing Huey · San Francisco, US
• Antoine M. Snijders · Antioch, US

Key dates

• Filing date: Apr 20, 2016
• Grant date: Nov 7, 2017
• Expiry date: Apr 20, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6809
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome).