Methods and systems for pure dye instrument normalization
US9809849B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 5, 2016 |
| Grant date | Nov 7, 2017 |
| Priority date | — |
| Expiry date | Feb 5, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12746
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.