Methods for retrieval of sequence-verified DNA constructs
US9809904B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Apr 23, 2012 |
| Grant date | Nov 7, 2017 |
| Priority date | — |
| Expiry date | Apr 23, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In some embodiments, methods of recovering a sequence-verified target nucleic acid are provided. In some embodiments, such methods may include tagging each member of a nucleic acid library with a set of adaptor sequences; sequencing the tagged members of the nucleic acid library; and recovering the sequence-verified target nucleic acid from the tagged and sequenced members of the nucleic acid library using a dial-out selection method. In certain embodiments, the members of the nucleic acid library may be tagged with a second set of adaptor sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.