High-throughput platform comprising microtissues perfused with living microvessels

US9810685B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Steven C. George · Chesterfield, US
• Christopher C. W. Hughes · Irvine, US
• Abraham P. Lee · Irvine, US
• Monica Moya · Irvine, US
• Yu-Hsiang Hsu · Pasadena, US

Key dates

• Filing date: Oct 5, 2011
• Grant date: Nov 7, 2017
• Expiry date: Oct 5, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2535/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided is a process for creating a 3D metabolically active microtissue perfused with living microvessels which have a direct fluidic connection with neighboring microfluidic channels. The process comprises preparing a template comprising a plurality of channels, and creating a network within said channels, said network comprising microfluidic channels, metabolically active living microvessels, and microtissues. The microvessels can sprout from said microvessels and/or form within the microtissue in response to a stimulus applied from said microfluidic channels or stimulus derived from the said tissues. In another embodiment, a device is provided comprising a supportive structure, one or more microfluidic channels, one or more microtissue compartments, and one or more microvessels, whereby the microvessels connect said microfludic channels and microtissue and perfuse the microtissue to deliver fluid from the microfluidic channels to the microtissues.